Our cell engineering and Development services cater to both tool or reagent and biological product development needs. We have extensive expertise to generate recombinant or stable cell lines for complex and wide protein class targets such as:
RTKs GPCRs Ion channels Immune modulation ligands or receptors Fc-gamma receptors GPI-anchored TNF-receptor superfamily Cell adhesion molecules Transporters Co-receptors Reporter genes
We are well-versed in working with diverse cellular backgrounds, including established cancer cell lines and primary cells. Hard-to-transfect cell lines required for binding studies, functional assays, immunization in vitro or in vivo screening and efficacy evaluation are successfully engineered to achieve the desired attributes.
For biologics programs, we have experience in product development for mAbs, immune-fusion molecules and cytokines. Typical activities include transfection, selection, limiting dilution, media/feed screening, cell line characterization, research cell banking and protein production.
Cell Lines
- Membrane-bound receptor cell lines with expression calibrated clones (low, medium and high)
- Receptor density measured and clones selected as per end-use requirement
- Knock-In (KI), Knock-Down (KD) and Knock-Out (KO) cell lines using siRNA / shRNA and CRISPR/Cas9 or TALEN technologies
- Single, double, or multiple gene KO / KD in primary and established cell lines
- Inducible cell line using Tet / Cumate inducible system
- Reporter cell lines (Fluorescent protein / Luciferase)
- Orthotopic cell line for in vivo xenograft and imaging applications
- Production cell lines using commercially available systems such as Freedom CHO-S/CHO DG44/CHOZN and in-house developed lentiviral system
Vector Systems
pcDNA series, pLVX and pGIPZ / pTRIPZ lentiviral vector series, pCHO1.0, pOptivec, pGL and in-house modified vector systems.
Transfection Methods
- Standard (lipid/nanoparticle)
- Electroporation
- Transposon
- Lentiviral transduction
Screening
- Flow cytometry
- Western blot
- PA-HPLC
- ELISA
- Octet
- ClonePix
- CellCelector
Quality Control
- Thaw test
- Mycoplasma
- Clonal stability
- Receptor density estimation
- SE-HPLC
- LC-MS analysis
Why Aurigene Cell Line Engineering and Development Services?
Strong framework data recording and traceability
Recombinant cell lines for wide protein classes
In-house lentiviral platform for challenging-to-express reagent proteins
Extensive experience working with various vector or cell line systems
Large inventory of cancer cell lines
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Learning Resources
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Gastro Retentive Dosage Form
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Cu-catalyzed coupling-cyclization in PEG 400 under ultrasound: a highly selective and greener approach towards isocoumarins
2013
The combination of CuI–K2CO3-PEG 400 facilitated the couplingcyclization of o-iodobenzoic acid with terminal alkynes under ultrasound, affording a greener and practical approach towards 3-substituted isocoumarins with remarkable regioselectivity. This inexpensive and Pd and ligand free methodology gave rise to various isocoumarins of potential pharmacological i...
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Development and assessment of a Bcs class II - SGLT2 (Sodium Glucose Cotransporter 2) inhibitor drug in the form of solid lipid Nanoparticles by selecting different lipids, co-surfactants, and manufacturing techniques
2005
Drug Delivery System (DDS) has been used successfully in the past few decades to cure illnesses and enhance health because of its improved systemic circulation and ability to regulate the drug's pharmacological action. As pharmacology and pharmacokinetics advanced, the idea of controlled release emerged, demonstrating the significance of drug release in assessing...
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Development of novel paullone-based PROTACs as anticancer agents
2005
Proteolysis-targeting chimera (PROTACs) represents a promising modality that has gained significant attention for cancer treatment. Using PROTAC technology, we synthesized novel structurally modified paullone-based PROTACs using Cereblon (CRBN) and Von Hippel–Lindau (VHL) E3 ligands....
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A simple RP-HPLC method development and verification of the Dissolution of Bromelain-A Complex mixture of proteolytic enzymes, in delayed release tablets
2005
Bromelain is a complex natural mixture of proteolytic enzymes derived from pineapple (Ananas cosmosus), which has good therapeutic properties. Bromelain can be found in all tissues of pineapple plants, including the stem, fruit, and leaves. Bromelain plays an important role in the treatment of many diseases such as soft tissue inflammation and edema, deep derma, ...
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