Our cell engineering services cater to both tool or reagent and biological product development requirements. We have strong expertise to generate recombinant or stable cell lines for complex and wide protein class targets such as:
RTKs GPCRs Ion channels Immune modulation ligands or receptors Fc-gamma receptors GPI-anchored TNF-receptor superfamily Cell adhesion molecules Transporters Co-receptors Reporter genes
We are well-versed in working with different cellular backgrounds such as established cancer cell lines and primary cells. Hard-to-transfect cell lines required for binding studies, functional assays, immunization in vitro or in vivo screening and efficacy evaluation applications are successfully engineered to achieve the desired attributes.
For biologics programs, we have experience of product development for mAbs, immune-fusion molecules and cytokines. The typical activities includes: transfection, selection, Limiting dilution, media/feed screening, cell line characterization, research cell banking and protein production
Cell Lines
- Membrane-bound receptor cell lines with expression calibrated clones (low, medium and high)
- Receptor density measured and clones selected as per end-use requirement
- Knock-In (KI), Knock-Down (KD) and Knock-Out (KO) cell lines using siRNA / shRNA and CRISPR/Cas9 or TALEN technologies
- Single, double or multiple gene KO / KD in primary and established cell lines
- Inducible cell line using Tet / Cumate inducible system
- Reporter cell lines (Fluorescent protein / Luciferase)
- Orthotopic cell line for in vivo xenograft and imaging applications
- Production cell lines using commercially available systems such as Freedom CHO-S/CHO DG44/CHOZN and in-house developed lentiviral system
- SARS-CoV-2 pseudo virus generation and neutralization assay
Vector Systems
pcDNA series, pLVX and pGIPZ / pTRIPZ lentiviral vector series, pCHO1.0, pOptivec, pGL and in-house modified vector systems
Transfection Methods
- Standard (lipid/nanoparticle)
- Electroporation
- Transposon
- Lentiviral transduction
Screening
- Flow cytometry
- Western blot
- PA-HPLC
- ELISA
- Octet
- ClonePix
Quality Control
- Thaw test
- Mycoplasma
- Clonal stability
- Receptor density estimation
- SE-HPLC
- LC-MS analysis
Why Aurigene Pharmaceutical Services?
Strong framework data recording and traceability
Recombinant cell lines for wide protein classes
In-house lentiviral platform for difficult to express reagent proteins
Extensive experience working with various vector or cell line systems
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Contact Us
Resources
JUNE 28, 2022
Neoantigen Specific T cells For Cancer Immunotherapy
Neoantigen specific T cells for cellular cancer immunotherapy An effective anti-tumor immune response in human is marked by presence of T cells reactive against neoantigens. Neoantigens are HLA-bound unique peptides arise from tumor-specific somatic mutations. Neoantigens are highly immunogenic because they are not present in normal tissues and hence...
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Building successful long-term partnerships with CDMOs from early drug discovery through commercialization
Building successful long-term partnerships with CDMOs from early drug discovery through commercialization Maximizing efficiency in drug research, development, and manufacturing is crucial for turning new innovations into therapeutic and financial benefits. Over the past couple of decades, pharmaceutical companies have increasingly turned to contr...
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Technology meets sustainability - How a complex API development process goes green
We are always looking for ways to enhance the sustainability of our products and services. Our team successfully developed a scalable manufacturing process for the API product of one of our Biotech clients using eco-friendly manufacturing technologies. Read the case study to learn more. ...
Read MoreAlternate end-game strategies towards Nirmatrelvir synthesis: Defining a continuous flow process for the preparation of an anti-COVID drug
2023
Scalable alternate end-game strategies for the synthesis of the anti-COVID drug molecule Nirmatrelvir (1,PF-07321332) have been described. The first involves a direct synthesis of 1 via amidation of the carboxylic acid 7 (suitably activated as a mixed anhydride with either pivaloyl chloride or T3P) with the ...
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Discovery of MAP855, an Efficacious and Selective MEK1/2 Inhibitor with an ATP-Competitive Mode of Action.
2021
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...
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Synthesis of Anti-covid Drug Nirmatrelvir Using Flow Chemistry
2021
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...
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Simple and efficient synthesis of imidazoquinoxalines and spiroquinoxalinones via pictect-spengler reaction using Wang resin, Synthetic Communications
2021
An efficient approach for the synthesis of various imidazoquinoxalines and spiroquinoxalinones has been reported from 2-(1H-imidazol-1-yl) aniline and .. ...
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