We characterize the potential genotoxicity of compounds using various test and assays:
In vivo micronucleus test Chromosomal aberration test Traditional Ames assay
The in vivo micronucleus assay (in vivo MNT) is a reliable and commonly-employed test to meet the regulatory requirements while submitting IND, to screen the compounds (such as chemicals, impurities, pesticides, insecticides, food and feed additives) for their clastogenic potential in bone marrow of rodents (rats/mice) and as a component of exposure-based risk assessment. This assay plays a vital role in adding weight of evidence in the hazardous measurement of any chemical and quantitative risk assessments.
The Chromosomal Aberration Test (CAT) is routinely employed as part of NPD and to meet regulatory expectation to screen the compounds (such as chemicals, impurities, pesticides, insecticides, food and feed additives) for their clastogenic potential to cells (human blood lymphocytes or mammalian cell lines in culture) by evaluating the development gene/chromosome damage in the form of structural aberrations at chromosome level.
Ames test is a rapid and reliable bacterial assay used for evaluating a chemical's potential genotoxicity by measuring its ability to induce reverse mutations at selected loci of modified Salmonella and E. coli bacterial strains. These strains have various mutations and are not capable of synthesizing an essential amino acid, either histidine (Salmonella) or tryptophan (E. coli), so they can only grow in the culture medium that is supplemented with that amino acid. Once the bacteria are exposed to a mutagen, mutation(s) occur that could restore/reverse the ability of the bacteria to synthesize the amino acid and to continue growth even after depletion of traces of provided amino acid in the agar. Relevant mutations involve substitution of individual base pairs or frameshift mutations caused by addition or deletion of a stretch of DNA. The Ames test can be carried out in the presence and absence of a metabolizing system to identify potential mutagenicity by the parent compound and/or its metabolites.
Quick turnaround time
Extensive experience in the genetic toxicology studies
Team of expert analytical and toxicology scientists
State-of-the-art facilities
MAY 19, 2023
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