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Cell Therapy Services Cell Therapy Services

Cell Therapy Services

We offer an integrated CAR T-cell therapy research and development services ranging from antibody discovery, novel CAR designing, pre-clinical studies to clinical development.

Chimeric Antigen Receptor T (CAR T) are T-cells that are genetically engineered and are used in cell therapy. Our expert team of scientists is committed to provide a wide range of services in CAR T-cell therapy research.

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CAR Designing

  • CAR discovery (scFv / vHH) and screening
  • 2nd / 3rd / 4th generation CARs designing (ScFV /vHH / linkers/co-stimulatory domain testing / safety switches / standard designs)
  • Dual/bi-specific CAR and universal / allogeneic CARs

CAR T Generation

  • Viral vector system (lentivirus/retrovirus)
  • Non-viral system (synthetic mRNA/ transposon)
  • Transduction/transfection of primary T-cells from healthy donors

CAR T Manufacturing Process Optimization

  • Media, cytokines and growth factors screening for ex vivo CAR T-cell manufacturing process optimization

CAR T Characterization

  • CAR detection (% CAR+ T-cells) by flow cytometry
  • Vector copy number (VCN) by ddPCR/ qRT-PCR
  • Immunophenotyping (%CD3+/%CD4+, (%CD3+/%CD8+ CAR-T cells), %TN, %TSCM, %TCM, %TEM and %TE during and after ex vivo CAR-T expansion
  • Activation (CD25) and exhaustion marker (PD-1, LAG-3, TIM3, CTLA-4) analysis

In vitro CAR T Screening

  • CAR-T proliferation (recombinant protein or co-culture (E:T) assay)
  • Cytotoxicity assay /co-culture (different E:T ratio) using target expressing parental or reporter cell lines
  • T-cell activation (CD25) marker analysis
  • T-cell exhaustion marker analysis (PD-1, LAG-3, TIM3, CTLA-4)
  • Inflammatory cytokine profiling (IL-2, IFN-y and TNF-a, CD107 a/b)

In vivo CAR T Efficacy

  • Tumor clearance study in orthotopic systemic NOD-SCID xenograft model (B-cell malignancies)
  • Immunophenotyping of circulation CAR-T cells
  • CAR-T activation, proliferation and persistence assessment by flow cytometry and Vector Copy Number (VCN) analysis
  • Cytokine profiling (IL-2, IFN-y and TNF-a)

Reagent Generation for CAR T

  • Lentivirus, retrovirus, synthetic mRNA, transposon system, KI/KO cell lines and reagent proteins

Why Aurigene Cell Therapy Services?

CAR discovery

CAR designing and screening

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Connect with our scientific experts for your drug discovery, development, and manufacturing needs

We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.

Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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