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Viral Vectors Services Viral Vectors Services

Viral Vectors Services

We deliver high-quality, research-grade Lentiviral (LV) and Adeno-associated viral (AAV) vectors to support preclinical cell and gene therapy (CGT) product development.

Viral vectors are the most effective method for gene transfer when modifying specific cell types or tissues, enabling the expression of therapeutic genes.  

We deliver high-quality research-grade Lentiviral (LV) and Adeno-associated viral (AAV) vectors to support preclinical cell and gene therapy (CGT) product development. 

Speak to our experts

Lentiviral (LV) Vector:

Plasmid production and characterization

  • GMP-like (VSV-G, Gag-Pol, Rev, and transfer plasmids)
  • QC by digestion and sequencing
  • Endotoxin-free

Upstream process

  • Co-transfection of VSV-G, Gag-Pol, Rev, and transfer plasmids
  • HEK293 cells (suspension or adherent)
  • Transfection (PEI or other methods)
  • Shake flask and wave bioreactors

Purification

  • Clarification, concentration, Benzonase treatment, and TFF
  • Ultracentrifuge or chromatography (affinity or AEX)
  • Concentration and buffer exchange, TFF, and sterile filtration

Analytics and release assays

  • Titer: p24 ELISA, qRT-PCR, HPLC, and functional titer (GFP or CAR)
  • Identity: qRT-PCR, GOI expression by flow cytometry or western blot analysis
  • Aggregation: DLS, SE-HPLC
  • Purity: SDS-PAGE, AEX-HPLC
  • Potency: Transduction of HEK293 cells for GOI expression

Adeno-associated Viral (AAV) Vector:

Plasmid production and characterization

  • GMP-like (Rep-Cap, helper, and transfer plasmids)
  • QC by digestion and sequencing
  • Endotoxin-free

Upstream process

  • Helper-free triple transfection
  • HEK293 cells (suspension or adherent)
  • Transfection (PEI or other methods)
  • Shake flask and wave bioreactors

Purification

  • Lysis, clarification and concentration, Benzonase treatment, TFF
  • Ultracentrifuge or chromatography (affinity, AEX)
  • Concentration and buffer exchange, TFF, and sterile filtration

Analytics and release assays

  • Titer: CP/mL by ELISA, VG/mL by qRT-PCR, HPLC, and functional titer
  • Identity: western blot analysis (VP1, VP2, & VP3) & ELISA, GOI expression by flow cytometry or western blot analysis
  • Aggregation: SE-HPLC
  • Purity: SDS-PAGE, SE-HPLC, & RP-HPLC
  • E/F ratio: AEX-HPLC, VG/CP ratio, A260/A280 ratio, and TEM
  • Potency: Transduction of HEK293 cells for GOI expression

Why Aurigene Pharmaceutical Viral Vectors Services?

State-of-the-art facilities

Team of scientists with extensive experience in viral vector generation

Virtual Tour

 

Connect with our scientific experts for your drug discovery, development, and manufacturing needs

We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.

Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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