A robust antibody discovery platform is an important core factor that determines the success of a set of drug modalities. The area of antibody discovery has seen tremendous technological advancement, offering pharma companies a wide range of tools to choose from. At Aurigene, we design our monoclonal antibody discovery programs keeping the therapeutic end point in mind and choose the technology platform accordingly.
Our team has experience in handling several technology platforms including humanized rodents, B-cell cloning, hybridoma, and display libraries. Fit-for-purpose tools and reagent monoclonal antibodies are generated to meet the program needs.
Antigen generation and Immunization strategies
- RNA, membrane and whole cell immunization
- Immunization with conjugate antigens
- Immunization with whole-cell, recombinant cell lines, nano disc and membrane preparations
- Immunization protocols; standard, RImMS and hybrid
- Neonatal strategy for better epitope specificity and less immunogenic epitope
- Display technologies: phage, yeast and mammalian: Under development
- Strong expertise in anti-idiotypic antibody discovery (blockers/non-blockers with single digit nM/pM affinity) to support PK / ADA assays
Hybridoma and B-Cell cloning
- Antigen-specific single B-cell sorting, sequencing / v-gene recovery, sub-cloning / reformatting, expression, screening and monoclonal selection
- Enriched B-cell or target-specific B-cell fusion methodologies
- Highly efficient and optimized fusion (PEG and electrofusion) technologies for generating hybridoma
Screening & evaluation strategies
- Multitier high-throughput screening strategies using flow cytometry, ELISA, and octet
- Analytical SE-HPLC, BIAcore, functional assay and mass spectrometry to support analytics
- Functional assay & fit-for-purpose assay (IHC, Western blotting etc.) for evaluation
Why Aurigene Pharmaceutical Services?
Single B-cell cloning
Humanized rodent platform
Display platforms (Phage/ Yeast)
Hybridoma
Contact Us
Resources
JUNE 28, 2022
Neoantigen Specific T cells For Cancer Immunotherapy
Neoantigen specific T cells for cellular cancer immunotherapy An effective anti-tumor immune response in human is marked by presence of T cells reactive against neoantigens. Neoantigens are HLA-bound unique peptides arise from tumor-specific somatic mutations. Neoantigens are highly immunogenic because they are not present in normal tissues and hence...
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Building successful long-term partnerships with CDMOs from early drug discovery through commercialization
Building successful long-term partnerships with CDMOs from early drug discovery through commercialization Maximizing efficiency in drug research, development, and manufacturing is crucial for turning new innovations into therapeutic and financial benefits. Over the past couple of decades, pharmaceutical companies have increasingly turned to contr...
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Technology meets sustainability - How a complex API development process goes green
We are always looking for ways to enhance the sustainability of our products and services. Our team successfully developed a scalable manufacturing process for the API product of one of our Biotech clients using eco-friendly manufacturing technologies. Read the case study to learn more. ...
Read MoreAlternate end-game strategies towards Nirmatrelvir synthesis: Defining a continuous flow process for the preparation of an anti-COVID drug
2023
Scalable alternate end-game strategies for the synthesis of the anti-COVID drug molecule Nirmatrelvir (1,PF-07321332) have been described. The first involves a direct synthesis of 1 via amidation of the carboxylic acid 7 (suitably activated as a mixed anhydride with either pivaloyl chloride or T3P) with the ...
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Discovery of MAP855, an Efficacious and Selective MEK1/2 Inhibitor with an ATP-Competitive Mode of Action.
2021
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...
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Synthesis of Anti-covid Drug Nirmatrelvir Using Flow Chemistry
2021
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...
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Simple and efficient synthesis of imidazoquinoxalines and spiroquinoxalinones via pictect-spengler reaction using Wang resin, Synthetic Communications
2021
An efficient approach for the synthesis of various imidazoquinoxalines and spiroquinoxalinones has been reported from 2-(1H-imidazol-1-yl) aniline and .. ...
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