The cardiac potassium channel, hERG is responsible for a rapid delayed rectifier current (IKr), which is primarily responsible for cardiac Action Potential (AP) repolarization. The unique structural properties of hERG make it vulnerable to interaction and inhibition by large numbers of compounds. Inhibition of IKr is the most common cause of cardiac AP prolongation by non-cardiac drugs. Prolonged AP causes prolongation of QT interval and has been associated with fatal ventricular arrhythmia, and Torsade de Pointes - which has led to a number of drug withdrawals and failures.
We conduct a Functional Observational Battery (FOB) test for the assessment of neurobehavioral changes induced by the test compound. Typically, various graded concentrations of test articles are formulated in suitable vehicles and administered to rats or mice. Studies are conducted as a single exposure regimen or as part of repeat dose toxicity studies. The pre and post-dose FOB measurements on the same day are made to evaluate the intra and intergroup comparison of effects.
Our scientists have vast experience in handling various test modules. The studies are conducted in full GLP compliance and data are reviewed by the Quality Assurance team.
In vitro assessment of new chemical entities (NCEs) for the effects on hERG potassium channels is an early indicator of possible QT prolonging effect. Patch clamp-electrophysiology is the gold standard for such an assessment. In addition to patch clamp assay, we also offer kit-based hERG assay for the preliminary screening of compounds.
|Reactivity to handling||Exophthalmos|
|Fur appearance||Pupil size|
|Defecation and Urination||Rearing|
|Somatosensory/ touch response||Tail pinch response|
|Visual approach response||Palpebral reflex|
|Pupillary light reflex||Righting reflex|
|Auditory startle reflex||--|
Motor activity is measured using an automated motor activity measuring system (OptoM3 activity meter with CI Multi device interface, Columbus Instruments, USA). Animal activity will be measured for a period of 30 minutes for each animal.
The grip strength meter measures the maximal muscle strength of the forelimb and hindlimb. For each animal, three readings will be recorded for forelimb and hindlimb using a Grip Strength Meter (Columbus Instruments, USA).
Body temperature: Rectal temperature will be recorded at the end of FOB observations using a digital thermometer.
Quick turnaround time
Extensive experience in the hERG assay & GLP FOB studies
Team of expert scientists
FEBRUARY 23, 2021
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