mRNA Synthesis IVT (In vitro Transcription)

We provide customized, high-quality and scalable mRNA production by in vitro transcription (IVT).

The services are integrated with high-quality plasmid production and discovery scale mRNA LNP formulation.

We offer a customized synthesis of mRNA for screening to preclinical studies. Our high-quality mRNA transcripts are ideal for functional studies, therapeutics, diagnostics and vaccine production. IVT is the method of choice to produce long and stable RNA molecules such as mRNA, guide RNA, SaRNA or long RNA when chemical synthesis is not possible.

Irrespective of scale, mRNA molecules go through rigorous in-process and final quality checks through a set of standard and customized analytical methods. Our mRNA processes are flexible and can be customized as per client needs.

Our mRNA services are integrated to high-quality plasmid DNA production, processing enzymes and other reagent generations. We provide high-throughput codon-optimized gene synthesis and cloning. We perform E. coli clone development, cell banking, E. coli fermentation and plasmid purification.

mRNA formulation: We support small scale mRNA formulation development and characterization.

Speak to our experts

In vitro transcription

Sequence design, gene synthesis and cloning
RNA transcripts from plasmids, PCR products and cDNA
Conventional and Self-amplifying RNA (SaRNA)
Flexibility to choose transcriptional promoter (T7 or SP6)
Normal unmodified mRNA synthesis
Use of modified bases in mRNA transcripts (Pseudo UTP etc.)
Several strategies for 5’ capping such as co-translational capping with Anti Reverse Cap Analog (ARCA)
Post-translational vaccinia capping
Options to choose from Cap0 and Cap1
Template-derived poly A tail or enzymatic incorporation in the IVT mRNA template
Optimization of mRNA translation and stability

Purification

Well-characterized in RNAse free environment
High-quality plasmid purification, characterization, and IVT template preparation
Method optimization for gram quantity
Plasmid DNA purification and characterization
Purification using lithium chloride precipitation and chromatography

Analytical control

Batch-to-batch consistency of the IVT mRNA
Bioanalyzer
Purity (tape station)
Length confirmation
Sequence confirmation by cDNA
Capping efficiency by mass-spec
Endotoxin measurement
SE-HPLC
DOT-BLOT
Immunogenicity testing for vaccine candidates

Why Aurigene mRNA Synthesis IVT (In vitro Transcription)?

Customized screening and scaling up strategies

In vitro transcription, purification and quality control

Customized formulation

Modernized state-of-the-art facility

Adaptable, scalable and high-quality

Other Services

Antibody Drug Conjugate (ADCs) Services

Cell Therapy Services

Monoclonal Antibody (mAb) Discovery Services

Viral Vectors Services

Virtual Tour

Hyderabad R and D Center

Bangalore R and D Center

Connect with our scientific experts for your drug discovery, development, and manufacturing needs

We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.

Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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