We offer a customized synthesis of mRNA for screening to preclinical studies. Our high-quality mRNA transcripts are ideal for functional studies, therapeutics, diagnostics and vaccine production. IVT is the method of choice to produce long and stable RNA molecules such as mRNA, guide RNA, SaRNA or long RNA when chemical synthesis is not possible.
Irrespective of scale, mRNA molecules go through rigorous in-process and final quality checks through a set of standard and customized analytical methods. Our mRNA processes are flexible and can be customized as per client needs.
Our mRNA services are integrated to high-quality plasmid DNA production, processing enzymes and other reagent generations. We provide high-throughput codon-optimized gene synthesis and cloning. We perform E. coli clone development, cell banking, E. coli fermentation and plasmid purification.
mRNA formulation: We support small scale mRNA formulation development and characterization.
In vitro transcription
- Sequence design, gene synthesis and cloning
- RNA transcripts from plasmids, PCR products and cDNA
- Conventional and Self-amplifying RNA (SaRNA)
- Flexibility to choose transcriptional promoter (T7 or SP6)
- Normal unmodified mRNA synthesis
- Use of modified bases in mRNA transcripts (Pseudo UTP etc.)
- Several strategies for 5’ capping such as co-translational capping with Anti Reverse Cap Analog (ARCA)
- Post-translational vaccinia capping
- Options to choose from Cap0 and Cap1
- Template-derived poly A tail or enzymatic incorporation in the IVT mRNA template
- Optimization of mRNA translation and stability
Purification
- Well-characterized in RNAse free environment
- High-quality plasmid purification, characterization, and IVT template preparation
- Method optimization for gram quantity
- Plasmid DNA purification and characterization
- Purification using lithium chloride precipitation and chromatography
Analytical control
- Batch-to-batch consistency of the IVT mRNA
- Bioanalyzer
- Purity (tape station)
- Length confirmation
- Sequence confirmation by cDNA
- Capping efficiency by mass-spec
- Endotoxin measurement
- SE-HPLC
- DOT-BLOT
- Immunogenicity testing for vaccine candidates
Why Aurigene Pharmaceutical Services?
Customized screening and scaling up strategies
In vitro transcription, purification and quality control
Customized formulation
Modernized state-of-the-art facility
Adaptable, scalable and high-quality
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Hyderabad R and D Center
Bangalore R and D Center
Resources
JUNE 28, 2022
Neoantigen Specific T cells For Cancer Immunotherapy
An effective anti-tumor immune response in human is marked by presence of T cells reactive against neoantigens. Neoantigens are HLA-bound unique peptides arise from tumor-specific somatic mutations. Neoantigens are highly immunogenic because they are not present in normal tissues and hence bypass central thymic tolerance. The success of immune checkpoint blockade...
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Peptide Development and Manufacturing
Peptides are short chains of amino acids that are linked by peptide bonds. Several peptides linked together are called polypeptides. A protein contains one or more polypeptides. Therefore, proteins are long chains of amino acids held together by peptide bonds....
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Cell Line Development
We enable development of stable and high yielding recombinant Mammalian and Microbial lines. ...
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Fixed Dose Combination Formulation
Introduction: A fixed dose combination (FDC) includes two or more active pharmaceutical ingredients (APIs) combined in a single dosage form. Fixed dose combination (FDC) product is expected to provide below advantages: Improved medication compliance by reducing the pill burden of patients. To achieve synergistic activity If combinations include doses of each drug...
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An efficient and convenient protocol for the synthesis of tetracyclic isoindolo[1,2-a]quinazoline derivatives
2016
A convenient and one-pot synthesis of tetracyclic isoindolo [1,2-a]quinazoline derivatives via Lewis acid mediated sequential C–N bond formation reactions is reported. This protocol provides a simple and rapid strategy for the synthesis of 12-benzylidene-10,12-dihydroisoindolo[1,2-b]quinazoline derivatives. However, a variety of tetracyclo indole fused quinazol...
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Alternate end-game strategies towards Nirmatrelvir synthesis: Defining a continuous flow process for the preparation of an anti-COVID drug
2005
Scalable alternate end-game strategies for the synthesis of the anti-COVID drug molecule Nirmatrelvir (1,PF-07321332) have been described. The first involves a direct synthesis of 1 via amidation of the carboxylic acid 7 (suitably activated as a mixed anhydride with either pivaloyl chloride or T3P) with the ...
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Discovery of MAP855, an Efficacious and Selective MEK1/2 Inhibitor with an ATP-Competitive Mode of Action.
2005
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...
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Synthesis of Anti-covid Drug Nirmatrelvir Using Flow Chemistry
2005
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...
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