We provide Plasmid DNA production service. We provide both research-grade and GMP-ready plasmids.
Research-grade plasmids are used for in vitro applications in cloning, mutagenesis, microbial transformation, and protein production.
GMP-ready plasmids are used in: IVT mRNA Protein and mAb production Stable cell line generation Bacmid generation Viral packaging Vaccine Gene therapy studies Animal immunization
Production scale: Small-scale (0.1 to 1 mg) Medium-scale (up to 10 mg) Large-scale (100 mg to 1 g)
Fermentation is done using E. coli. The cells are harvested via centrifugation etc.
For extraction, lysis is done in two ways: alkaline lysis or mechanical lysis. Plasmid DNA pre-purification is done using Tangential Flow Filtration (TFF).
Purification is done using ion exchange/size exclusion chromatography & polishing. Further purification is done using TFF.
Small-scale and large-scale manufacturing
High quality (mg to g scale) production
Both research grade and GMP ready plasmid production
JUNE 28, 2022
Neoantigen specific T cells for cellular cancer immunotherapy An effective anti-tumor immune response in human is marked by presence of T cells reactive against neoantigens. Neoantigens are HLA-bound unique peptides arise from tumor-specific somatic mutations. Neoantigens are highly immunogenic because they are not present in normal tissues and hence...Read More
JANUARY 04, 2021
Building successful long-term partnerships with CDMOs from early drug discovery through commercialization Maximizing efficiency in drug research, development, and manufacturing is crucial for turning new innovations into therapeutic and financial benefits. Over the past couple of decades, pharmaceutical companies have increasingly turned to contr...Read More
We are always looking for ways to enhance the sustainability of our products and services. Our team successfully developed a scalable manufacturing process for the API product of one of our Biotech clients using eco-friendly manufacturing technologies. Read the case study to learn more. ...Read More
Scalable alternate end-game strategies for the synthesis of the anti-COVID drug molecule Nirmatrelvir (1,PF-07321332) have been described. The first involves a direct synthesis of 1 via amidation of the carboxylic acid 7 (suitably activated as a mixed anhydride with either pivaloyl chloride or T3P) with the ...Read More
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...Read More
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...Read More
An efficient approach for the synthesis of various imidazoquinoxalines and spiroquinoxalinones has been reported from 2-(1H-imidazol-1-yl) aniline and .. ...Read More
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