We provide Plasmid DNA production service. We provide both research-grade and GMP-ready plasmids.
Research-grade plasmids are used for in vitro applications in cloning, mutagenesis, microbial transformation, and protein production.
GMP-ready plasmids are used in: Stable cell line generation Bacmid generation Viral packaging Vaccine Gene therapy studies Animal immunization
Production scale: Small-scale (0.1 to 1 mg) Medium-scale (up to 10 mg) Large-scale (100 mg to 1 g)
Fermentation is done using E. coli. The cells are harvested via centrifugation etc.
For extraction, lysis is done in two ways: alkaline lysis or mechanical lysis. Plasmid DNA pre-purification is done using Tangential Flow Filtration (TFF).
Purification is done using ion exchange/size exclusion chromatography & polishing. Further purification is done using TFF.
Small-scale and large-scale manufacturing
High quality (mg to g scale) production
Both research grade and GMP ready plasmid production
JUNE 28, 2022
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