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Plasmid Development and Manufacturing Services Plasmid Development and Manufacturing Services

Plasmid Development and Manufacturing Services

We offer high-quality research-grade and GMP-ready plasmids.

We provide Plasmid DNA production service. We provide both research-grade and GMP-ready plasmids.     

Research-grade plasmids are used for in vitro applications in cloning, mutagenesis, microbial transformation, and protein production.     

GMP-ready plasmids are used in: IVT mRNA Protein and mAb production Stable cell line generation Bacmid generation Viral packaging Vaccine Gene therapy studies Animal immunization     

Production scale: Small-scale (0.1 to 1 mg) Medium-scale (up to 10 mg) Large-scale (100 mg to 1 g)

Speak to our experts

Fermentation

Fermentation is done using E. coli. The cells are harvested via centrifugation etc.

Extraction

For extraction, lysis is done in two ways: alkaline lysis or mechanical lysis. Plasmid DNA pre-purification is done using Tangential Flow Filtration (TFF).

Purification and Formulation

Purification is done using ion exchange/size exclusion chromatography & polishing. Further purification is done using TFF.

Plasmid scale-up

Small-scale and large-scale manufacturing

  • Typical yields: 2-5 mg/L (medium to high copy plasmids)
  • Scale: 10-20 L shake flask, 5-20 L fermentation
  • Typical timelines: 2-3 weeks for RG and 6-8 weeks for GLP ready

Why Aurigene Pharmaceutical Services?

High quality (mg to g scale) production

Both research grade and GMP ready plasmid production

Virtual Tour

 

Connect with our scientific experts for your drug discovery, development, and manufacturing needs

We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.

Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.

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