For most of the large molecules and bio reagent projects, molecular biology is the starting point which involves construct design, gene synthesis and cloning. In addition to standard molecular biology solutions, we provide specialized services to support target identification, antibody discovery, metabolic engineering and translational research.
Our service range includes:
Recombinant DNA technology Antibody gene sequencing (high-throughput) Mutagenesis Customized Library generation Plasmid DNA preparation & scale-up for CGT requirements Microbial genome engineering Gene expression analysis IVT mRNA synthesis
Recombinant DNA Technology
Cloning and vector generation:
Gene cloning service at Aurigene supports customized generation of recombinant constructs by various methods for generation of antibodies, enzymes, and reagent proteins:
- Ligation dependent and independent cloning
- Recombination based high throughput cloning
- De novo cloning (synthetic gene fragments, PCR, and cDNA based)
- Customized vector construction based on client requirement
Plasmid DNA preparation:
Plasmid DNA purification service caters to both small research facilities as well as large-scale manufacturing for biotech and pharmaceutical companies:
- Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production
- High-quality industrial grade plasmid suitable for :
- Protein
- Antibody production
- Stable cell line generation in mammalian cells
- Bacmid generation for protein expression in insect cells
- Viral packaging
- In vitro mRNA synthesis
- Vaccine
- Gene therapy studies, and
- Animal immunization
- Process development for customized plasmid propagation and purification
Mutagenesis:
Site-directed mutagenesis service enables life science researchers by creating specific DNA mutations for studying gene regulation, DNA-protein interactions, protein structure or function relationship, enzyme function and novel recombinant proteins:
- Point mutation, insertion, deletion or gene fragment replacement
- Customized mutagenesis requirements such as random and site-directed mutagenesis and mutant library generation
- Gene shuffling, combinatorial mutagenesis, alanine scanning and site saturation mutagenesis
Antibody Gene Sequencing and Reformatting
Antibody sequence information from hybridoma is essential for protection, preservation and optimization of monoclonal antibodies (mAbs). Antibody gene sequencing and reformatting at Aurigene is a one-stop solution for recombinant expression and production of antibodies:
- RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation
- Degenerate oligos
- De novo antibody sequencing and analysis using LC-MS/MS
- Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, and VHH or nanobody
- Antibody gene sequencing for mouse, rat, rabbit and other species.
Strain Engineering
Experience in genome scale modification of bacterial and yeast strains for production of recombinant proteins, platform chemicals and metabolic engineering:
- BSL-1 and BSL-2 microbes
- Gene knock out and knock-in using homologous recombination based methods
- Characterization of engineered strains using microbiological methods, RT-PCR and advanced analytics
- CRISPR-Cas9 based mutagenesis
Library Generation and Evaluation
Constructing cDNA libraries, phage libraries and yeast libraries along with provisions for high throughput screening and functional characterization.
Gene expression analysis
Regulation and expression of genes at the transcriptional and translational stages play a crucial role in determining gene function. Scientists at Aurigene carry out routine expression studies by several methods such as:
- Quantitative real time PCR
- ELISA in various formats
- Flow cytometry
- Western blotting
- Mass spectrometry
Residual Protein, DNA and Mycoplasma Detection
Residual host-cell proteins (HCP) and host-cell DNA (HCD) are impurities that remain in recombinant bio therapeutics. We ensure that these impurities and contaminants are detected and reduced to levels below those guided by regulatory agencies:
- Quantitative real-time PCR
- ELISA
- SPR (BIAcore)
- Mycoplasma test
Why Aurigene Molecular Biology Services?
State-of-the-art facilities with high-end instrumentation
Wide range of tools under one umbrella
Customized generation of recombinant constructs
Fast and innovative solutions
One-stop solution for recombinant expression and production of antibodies
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Learning Resources
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Biologics Formulation Development Services
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Systematic formulation design - shorten development cost & time
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A metal catalyst-free and one-pot synthesis of (3,4- dihydro-2H-benzo[b][1,4]oxazin-2-yl)methanol derivatives in water
2015
A robust and metal catalyst-free method has been developed for the general and green synthesis of racemic (3,4-dihydro-2H-benzo[b][1,4] oxazin-2-yl)methanol derivatives. This simple, mild and practical method involves the reaction of 2-aminophenols with ()-epichlorohydrin in the presence of NaOH in water at room temperature. The reaction features high regioselec...
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Development of novel paullone-based PROTACs as anticancer agents
Proteolysis-targeting chimera (PROTACs) represents a promising modality that has gained significant attention for cancer treatment. Using PROTAC technology, we synthesized novel structurally modified paullone-based PROTACs using Cereblon (CRBN) and Von Hippel–Lindau (VHL) E3 ligands....
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Development and verification of RP-HPLC method for the quantitative determination of Decitabine in tablet dosage formulation
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