For most of the large molecules and bio reagent projects, molecular biology is the starting point which involves construct design, gene synthesis and cloning. In addition to standard molecular biology solutions, we provide specialized services to support target identification, antibody discovery, metabolic engineering and translational research.
Our service range includes:
Recombinant DNA technology Antibody gene sequencing (high-throughput) Mutagenesis Customized Library generation Plasmid DNA preparation & scale-up for CGT requirements Microbial genome engineering Gene expression analysis IVT mRNA synthesis
Recombinant DNA Technology
Cloning and vector generation:
Gene cloning service at Aurigene supports customized generation of recombinant constructs by various methods for generation of antibodies, enzymes, and reagent proteins:
- Ligation dependent and independent cloning
- Recombination based high throughput cloning
- De novo cloning (synthetic gene fragments, PCR, and cDNA based)
- Customized vector construction based on client requirement
Plasmid DNA preparation:
Plasmid DNA purification service caters to both small research facilities as well as large-scale manufacturing for biotech and pharmaceutical companies:
- Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production
- High-quality industrial grade plasmid suitable for :
- Protein
- Antibody production
- Stable cell line generation in mammalian cells
- Bacmid generation for protein expression in insect cells
- Viral packaging
- In vitro mRNA synthesis
- Vaccine
- Gene therapy studies, and
- Animal immunization
- Process development for customized plasmid propagation and purification
Mutagenesis:
Site-directed mutagenesis service enables life science researchers by creating specific DNA mutations for studying gene regulation, DNA-protein interactions, protein structure or function relationship, enzyme function and novel recombinant proteins:
- Point mutation, insertion, deletion or gene fragment replacement
- Customized mutagenesis requirements such as random and site-directed mutagenesis and mutant library generation
- Gene shuffling, combinatorial mutagenesis, alanine scanning and site saturation mutagenesis
Antibody Gene Sequencing and Reformatting
Antibody sequence information from hybridoma is essential for protection, preservation and optimization of monoclonal antibodies (mAbs). Antibody gene sequencing and reformatting at Aurigene is a one-stop solution for recombinant expression and production of antibodies:
- RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation
- Degenerate oligos
- De novo antibody sequencing and analysis using LC-MS/MS
- Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, and VHH or nanobody
- Antibody gene sequencing for mouse, rat, rabbit and other species.
Strain Engineering
Experience in genome scale modification of bacterial and yeast strains for production of recombinant proteins, platform chemicals and metabolic engineering:
- BSL-1 and BSL-2 microbes
- Gene knock out and knock-in using homologous recombination based methods
- Characterization of engineered strains using microbiological methods, RT-PCR and advanced analytics
- CRISPR-Cas9 based mutagenesis
Library Generation and Evaluation
Constructing cDNA libraries, phage libraries and yeast libraries along with provisions for high throughput screening and functional characterization.
Gene expression analysis
Regulation and expression of genes at the transcriptional and translational stages play a crucial role in determining gene function. Scientists at Aurigene carry out routine expression studies by several methods such as:
- Quantitative real time PCR
- ELISA in various formats
- Flow cytometry
- Western blotting
- Mass spectrometry
Residual Protein, DNA and Mycoplasma Detection
Residual host-cell proteins (HCP) and host-cell DNA (HCD) are impurities that remain in recombinant bio therapeutics. We ensure that these impurities and contaminants are detected and reduced to levels below those guided by regulatory agencies:
- Quantitative real-time PCR
- ELISA
- SPR (BIAcore)
- Mycoplasma test
Why Aurigene Pharmaceutical Services?
State-of-the-art facilities with high-end instrumentation
Wide range of tools under one umbrella
Customized generation of recombinant constructs
Fast and innovative solutions
One-stop solution for recombinant expression and production of antibodies
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Resources
APRIL 24, 2023
The importance of business continuity planning in CRDMO industry
Both natural and unnatural catastrophic events inflict negative consequences due to the ever-increasing interconnectedness of the global economy. Those consequences are certain to last for longer duration. e.g.; The Covid-19 pandemic is still having a negative impact on the global economy. Maintaining continuity is critical for all businesses, but perhaps no othe...
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Accelerating Drug Discovery Through Innovative Partnerships
Genomics plays a vital role in identifying which gene is associated with a specific disease. A gene called CNOT1 is for example known for it's effect on brain development and for impairing memory and learning. Despite the great promise genomics provides in understanding the disease, genes are not the best drug targets....
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Biologics Formulation Development Services
Deep rooted in our fundamental understanding of protein chemistry, our formulation expertise spans a wide range of proteins such as – chemically derivatized proteins, receptor fusion proteins, hyperglycosylated proteins and monoclonal antibodies and fragments. ...
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Systematic formulation design - shorten development cost & time
Project Challenge: Existing formulation (lower strength - 50 mg) was manufactured using direct compression process. This formulation posed poor powder flow and content uniformity issues during scale-up. Development of new strength 150 mg without any process issues was a challenge. Solution design: Preliminary pre-formulation, stress study and PSD impact assessmen...
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A metal catalyst-free and one-pot synthesis of (3,4- dihydro-2H-benzo[b][1,4]oxazin-2-yl)methanol derivatives in water
2015
A robust and metal catalyst-free method has been developed for the general and green synthesis of racemic (3,4-dihydro-2H-benzo[b][1,4] oxazin-2-yl)methanol derivatives. This simple, mild and practical method involves the reaction of 2-aminophenols with ()-epichlorohydrin in the presence of NaOH in water at room temperature. The reaction features high regioselec...
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Alternate end-game strategies towards Nirmatrelvir synthesis: Defining a continuous flow process for the preparation of an anti-COVID drug
2005
Scalable alternate end-game strategies for the synthesis of the anti-COVID drug molecule Nirmatrelvir (1,PF-07321332) have been described. The first involves a direct synthesis of 1 via amidation of the carboxylic acid 7 (suitably activated as a mixed anhydride with either pivaloyl chloride or T3P) with the ...
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Discovery of MAP855, an Efficacious and Selective MEK1/2 Inhibitor with an ATP-Competitive Mode of Action.
2005
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...
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Synthesis of Anti-covid Drug Nirmatrelvir Using Flow Chemistry
2005
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...
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