Our team has experience in handling difficult and complex target molecules. We offer wide range of services and solutions with the flexibility of multiple entry points. We are proficient with most modern expression platforms and our facilities are equipped to handle scales ranging from mgs to grams.
Protein crystallography services provide a one-stop solution for determining 3D structure of: Proteins and complexes : Protein-protein complexes Protein-small molecule complexes Antibody-antigen complexes at high resolution
Protein crystallography projects are typically carried out in gene-to-structure format encompassing gene cloning, protein expression, purification, crystallization, screening, synchrotron x-ray diffraction, structure solution and refinement.
Our structural biology expertise also includes protein engineering to enhance crystallizability of difficult proteins. We seamlessly integrate structural biology with drug discovery programs for end-to-end SBDD offerings.
Expression Systems
Mammalian: Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc. Protein production in transient expression mode or using stable clones or pools.
Insect cell: Baculovirus mediated protein expression and production in Sf9, Sf21, Hi5 and super Sf9 insect cell lines
Yeast: Stable protein expression in Pichia, recombinant molecule is typically secreted in media by genome integration.
Bacterial: Soluble, periplasmic and inclusion bodies in E. coli. Multi-protein expression by co-transfection or duet plasmid technology. Refolding condition screening and method optimization. Construct design, scouting and screening studies for challenging proteins. Protein expression scale-up using spinner flask, shaker flask, HYPER flask and WAVE bioreactor platforms to handle volumes up to 50 L scale with low operating costs.
Protein Purification
Combination of high-end instruments and a wealth of scientific expertise we provide the best in class customized purification platforms for research and drug discovery needs. We are proficient in purifying tagged as well as un-tagged and all classes of target proteins from mammalian, yeast and microbial expression systems.
- Purification process optimization
- Protein purification in batch binding mode as well as automated FPLC systems
- Chromatographic purification methods include IMAC, IEC, mixed-mode, HIC, SEC and affinity for micrograms to gram-scale quantities
Antibody Purification
- Purification of monoclonal antibodies of different origins (human, non-human primates, mouse, rat, rabbit, pig, hamster, gerbil, etc.)
- Purification of antibody fragments of various formats (Fab, Fab2, F(ab)’2, VHH, scfv and generation from full length mAbs)
- Bi-specific and KIH antibodies
- Diabodies
- Single domain antibody
- Protein purification from hybridoma cultures
Microbial Purification Capabilities
- Soluble protein
- Periplasmic protein
- Intracellular protein
- Refolding from inclusion bodies
- Purification of Major Histocompatibility (MHC) complexes
- Membrane protein production – GPCRs, nanodisc technology, etc.
- Antibody drug conjugate screening, development and purification
- High throughput protein screening and purification
- Protein cleavage and purification
Protein Analytics and Characterization
We are committed to deliver high-quality products by performing a number of routine protein analyses throughout all project stages ensuring the identity, purity, structural and conformational integrity, stability and function of the protein.
- Quantitative analysis: Protein estimation using Absorbance at 280nm, Bradford and BCA assays
- Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blot analysis
- Purity: SE-HPLC, IEX-HPLC and RP-HPLC
- Protein stability analysis (Real-time and Accelerated Stability Testing)
- LC-MS analysis
- Capillary Electrophoresis (CE)
- Peptide Mass Fingerprinting (PMF)
- Biological activity and binding studies using enzymatic assays, ELISA, BIAcore etc.
- Endotoxin analysis
- Host Cell Protein (HCP) and Host Cell DNA (HCD) analysis
Protein Stability Analysis
- Real-time and accelerated stability testing
- Long-term stability studies
Protein Conjugation and Labeling
- Protein labeling/conjugation (HRP, biotin, Ruthenium, fluorescent labels etc.)
- Conjugation of resin with specific protein/peptide
Crystallization Screening
- Multi-dimensional optimization of crystallization conditions using commercial and customized screens for a target protein or complex
- Sitting drop, hanging drop, micro-batch (under oil) and seeding
- Micro-seeding and macro-seeding
Crystal Hit Identification
- Microscopic examination of drops, staining and mass spectromety of harvested crystals
- Preliminary X-ray diffraction
Protein-protein and Protein-drug Complexes
- Crystallization of protein-inhibitor/ligand complexes (co-crystallization)
- Soaking of crystals in inhibitor/ligand solution
Data Collection and Refinement
- Cryoprotectant screening
- Complete X-ray data collection using rotating anode source
- Synchrotron data collection at Australian synchrotron
- Molecular replacement and structure refinement
- Structure analysis and data presentation
Why Aurigene Pharmaceutical Services?
Multidisciplinary team of scientists
Scalability with high purity and exceptional quality
Processing complex molecules
Protein engineering for enhanced crystallizability
Synchrotron data collection
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Contact Us
Resources
JUNE 28, 2022
Neoantigen Specific T cells For Cancer Immunotherapy
Neoantigen specific T cells for cellular cancer immunotherapy An effective anti-tumor immune response in human is marked by presence of T cells reactive against neoantigens. Neoantigens are HLA-bound unique peptides arise from tumor-specific somatic mutations. Neoantigens are highly immunogenic because they are not present in normal tissues and hence...
Read More
Building successful long-term partnerships with CDMOs from early drug discovery through commercialization
Building successful long-term partnerships with CDMOs from early drug discovery through commercialization Maximizing efficiency in drug research, development, and manufacturing is crucial for turning new innovations into therapeutic and financial benefits. Over the past couple of decades, pharmaceutical companies have increasingly turned to contr...
Read More
Technology meets sustainability - How a complex API development process goes green
We are always looking for ways to enhance the sustainability of our products and services. Our team successfully developed a scalable manufacturing process for the API product of one of our Biotech clients using eco-friendly manufacturing technologies. Read the case study to learn more. ...
Read MoreAlternate end-game strategies towards Nirmatrelvir synthesis: Defining a continuous flow process for the preparation of an anti-COVID drug
2023
Scalable alternate end-game strategies for the synthesis of the anti-COVID drug molecule Nirmatrelvir (1,PF-07321332) have been described. The first involves a direct synthesis of 1 via amidation of the carboxylic acid 7 (suitably activated as a mixed anhydride with either pivaloyl chloride or T3P) with the ...
Read More-
Discovery of MAP855, an Efficacious and Selective MEK1/2 Inhibitor with an ATP-Competitive Mode of Action.
2021
Mutations in MEK1/2 have been described as a resistance mechanism to BRAF/MEK inhibitor treatment. We report the discovery of a novel ATP-competitive MEK1/2 inhibitor with efficacy in wildtype (WT) and mutant MEK12 models. Starting from a HTS hit, we obtained selective, cellularly active ...
Read More -
Synthesis of Anti-covid Drug Nirmatrelvir Using Flow Chemistry
2021
Synthesis of the anti-covid therapeutic Nirmatrelvir by using flow chemistry to enhance efficiency of amide to nitrile conversion in a functionally and Stereochemically Embellished environment. ...
Read More -
Simple and efficient synthesis of imidazoquinoxalines and spiroquinoxalinones via pictect-spengler reaction using Wang resin, Synthetic Communications
2021
An efficient approach for the synthesis of various imidazoquinoxalines and spiroquinoxalinones has been reported from 2-(1H-imidazol-1-yl) aniline and .. ...
Read More
You are about to leave Aurigene Pharmaceutical Services and affiliates website. Aurigene Pharmaceutical Services assumes no responsibility for the information presented on the external website or any further links from such sites. These links are presented to you only as a convenience, and the inclusion of any link does not imply endorsement by Aurigene Pharmaceutical Services.
If you wish to continue to this external website, click Proceed.