Our team has experience in handling difficult and complex target molecules. We offer wide range of services and solutions with the flexibility of multiple entry points. We are proficient with most modern expression platforms and our facilities are equipped to handle scales ranging from mgs to grams.
Protein crystallography services provide a one-stop solution for determining 3D structure of: Proteins and complexes : Protein-protein complexes Protein-small molecule complexes Antibody-antigen complexes at high resolution
Protein crystallography projects are typically carried out in gene-to-structure format encompassing gene cloning, protein expression, purification, crystallization, screening, synchrotron x-ray diffraction, structure solution and refinement.
Our structural biology expertise also includes protein engineering to enhance crystallizability of difficult proteins. We seamlessly integrate structural biology with drug discovery programs for end-to-end SBDD offerings.
Expression Systems
Mammalian: Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc. Protein production in transient expression mode or using stable clones or pools.
Insect cell: Baculovirus mediated protein expression and production in Sf9, Sf21, Hi5 and super Sf9 insect cell lines
Yeast: Stable protein expression in Pichia, recombinant molecule is typically secreted in media by genome integration.
Bacterial: Soluble, periplasmic and inclusion bodies in E. coli. Multi-protein expression by co-transfection or duet plasmid technology. Refolding condition screening and method optimization. Construct design, scouting and screening studies for challenging proteins. Protein expression scale-up using spinner flask, shaker flask, HYPER flask and WAVE bioreactor platforms to handle volumes up to 50 L scale with low operating costs.
Protein Purification
Combination of high-end instruments and a wealth of scientific expertise we provide the best in class customized purification platforms for research and drug discovery needs. We are proficient in purifying tagged as well as un-tagged and all classes of target proteins from mammalian, yeast and microbial expression systems.
- Purification process optimization
- Protein purification in batch binding mode as well as automated FPLC systems
- Chromatographic purification methods include IMAC, IEC, mixed-mode, HIC, SEC and affinity for micrograms to gram-scale quantities
Antibody Purification
- Purification of monoclonal antibodies of different origins (human, non-human primates, mouse, rat, rabbit, pig, hamster, gerbil, etc.)
- Purification of antibody fragments of various formats (Fab, Fab2, F(ab)’2, VHH, scfv and generation from full length mAbs)
- Bi-specific and KIH antibodies
- Diabodies
- Single domain antibody
- Protein purification from hybridoma cultures
Microbial Purification Capabilities
- Soluble protein
- Periplasmic protein
- Intracellular protein
- Refolding from inclusion bodies
- Purification of Major Histocompatibility (MHC) complexes
- Membrane protein production – GPCRs, nanodisc technology, etc.
- Antibody drug conjugate screening, development and purification
- High throughput protein screening and purification
- Protein cleavage and purification
Protein Analytics and Characterization
We are committed to deliver high-quality products by performing a number of routine protein analyses throughout all project stages ensuring the identity, purity, structural and conformational integrity, stability and function of the protein.
- Quantitative analysis: Protein estimation using Absorbance at 280nm, Bradford and BCA assays
- Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blot analysis
- Purity: SE-HPLC, IEX-HPLC and RP-HPLC
- Protein stability analysis (Real-time and Accelerated Stability Testing)
- LC-MS analysis
- Capillary Electrophoresis (CE)
- Peptide Mass Fingerprinting (PMF)
- Biological activity and binding studies using enzymatic assays, ELISA, BIAcore etc.
- Endotoxin analysis
- Host Cell Protein (HCP) and Host Cell DNA (HCD) analysis
Protein Stability Analysis
- Real-time and accelerated stability testing
- Long-term stability studies
Protein Conjugation and Labeling
- Protein labeling/conjugation (HRP, biotin, Ruthenium, fluorescent labels etc.)
- Conjugation of resin with specific protein/peptide
Crystallization Screening
- Multi-dimensional optimization of crystallization conditions using commercial and customized screens for a target protein or complex
- Sitting drop, hanging drop, micro-batch (under oil) and seeding
- Micro-seeding and macro-seeding
Crystal Hit Identification
- Microscopic examination of drops, staining and mass spectromety of harvested crystals
- Preliminary X-ray diffraction
Protein-protein and Protein-drug Complexes
- Crystallization of protein-inhibitor/ligand complexes (co-crystallization)
- Soaking of crystals in inhibitor/ligand solution
Data Collection and Refinement
- Cryoprotectant screening
- Complete X-ray data collection using rotating anode source
- Synchrotron data collection at Australian synchrotron
- Molecular replacement and structure refinement
- Structure analysis and data presentation
Why Aurigene Protein Sciences and Structural Biology Services?
Multidisciplinary team of scientists
Scalability with high purity and exceptional quality
Processing complex molecules
Protein engineering for enhanced crystallizability
Synchrotron data collection
Virtual Tour
Hyderabad R and D Center
Bangalore R and D Center
Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Learning Resources
The importance of business continuity planning in CRDMO industry
Both natural and unnatural catastrophic events inflict negative consequences due to the ever-increasing interconnectedness of the global economy. Those consequences are certain to last for longer duration. e.g.; The Covid-19 pandemic is still having a negative impact on the global economy. Maintaining continuity is critical for all businesses, but perhaps no othe...
Read More
HPAPI Molecule from Development to Market
The global Highly Potent Active Pharmaceutical Ingredients (HPAPI) market is expected to reach USD 26.84 Billion by 2023 from USD 17.72 Billion in 2018, at a CAGR 8.7%....
Read More
Biologics Process Development Services
Our strength is built on a deep understanding of cell culture, protein chemistry and an integrated analytics platform enabling a robust, scalable and controlled process. ...
Read More
Expediting early development through innovative precision capsule filling technology
Introduction: Any new chemical entity (NCE) needs to undergo various stages of development such as preclinical and clinical trials before drug product is approved by regulatory agencies and available for patient. Formulations developed during early phases are simple formulations to enable phase appropriate studies like screening, dose ranging, toxicological and d...
Read More
A metal catalyst-free and one-pot synthesis of (3,4- dihydro-2H-benzo[b][1,4]oxazin-2-yl)methanol derivatives in water
2015
A robust and metal catalyst-free method has been developed for the general and green synthesis of racemic (3,4-dihydro-2H-benzo[b][1,4] oxazin-2-yl)methanol derivatives. This simple, mild and practical method involves the reaction of 2-aminophenols with ()-epichlorohydrin in the presence of NaOH in water at room temperature. The reaction features high regioselec...
Read More-
A simple RP-HPLC method development and verification of the Dissolution of Bromelain-A Complex mixture of proteolytic enzymes, in delayed release tablets
2005
Bromelain is a complex natural mixture of proteolytic enzymes derived from pineapple (Ananas cosmosus), which has good therapeutic properties. Bromelain can be found in all tissues of pineapple plants, including the stem, fruit, and leaves. Bromelain plays an important role in the treatment of many diseases such as soft tissue inflammation and edema, deep derma, ...
Read More -
A sensitive and a simple RP-HPLC method development and verification for the quantitative estimation of Cholecalciferol in tablets
2005
Cholecalciferol, also known as Vitamin D3, is widely prescribed for the treatment osteomalacia and osteoporosis [1]. It also plays a key role in calcium and phosphorus homeostasis and skeletal mineralization [2]. IUPAC name of Cholecalciferol is (3β, 5Z, 7E) 9,10-secocholesta-5,7,10(19)-trien-3-ol, whose molecular weight is 384.64 g/mol and its molecular formula...
Read More -
A simple RP-HPLC method development and verification for the quantitative estimation of Sodium Butyrate in tablets
2005
Sodium butyrate is the sodium salt of butyric acid (Fig. 1), which exhibits various properties such as immune system modulator, antioxidant, and anti-inflammatory properties [1]. Furthermore, sodium butyrate is used as a promoter of growth in milk replacer formula for young calves [2]....
Read More
You are about to leave Aurigene Pharmaceutical Services and affiliates website. Aurigene Pharmaceutical Services assumes no responsibility for the information presented on the external website or any further links from such sites. These links are presented to you only as a convenience, and the inclusion of any link does not imply endorsement by Aurigene Pharmaceutical Services.
If you wish to continue to this external website, click Proceed.
Leaving already?
Don't forget to join us at
CPHI Worldwide 2023.
October 24th-26th, 2023 | Barcelona, Spain
Get ready to accelerate your drug’s journey to the market