The mammalian cell micronucleus test (MNT) is routinely employed as part of NPD and to meet regulatory expectation to screen the compounds (chemicals, impurities, pesticides, insecticides, food and feed additives etc) for their clastogenic potential to cells (human blood lymphocytes or mammalian cell lines in culture by evaluating the development gene/chromosome damage in the form of micronuclei.
Cells in cultures will exposed to the compound of interest at different concentrations for shorter duration (3-6 hours) and continuously (1.5 cell cycle time) till harvesting. In shorter duration exposure, testing will be performed by supplying liver metabolizing enzymes to evaluate by products of compound and in other hand absence of metabolism support in shorter and continuous exposures to evaluate direct compound activity.
Positive results manifested as increase in the frequency of micronucleus appearance in comparison with controls. Increase can be statistically significant and/or dose-dependent.
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