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Cell Line Engineering and Development Cell Line Engineering and Development

Cell Line Engineering and Development

Recombinant cell line development for wide range of protein classes.

Our cell engineering and Development services cater to both tool or reagent and biological product development requirements. We have strong expertise to generate recombinant or stable cell lines for complex and wide protein class targets such as:    

RTKs GPCRs Ion channels Immune modulation ligands or receptors Fc-gamma receptors GPI-anchored TNF-receptor superfamily Cell adhesion molecules Transporters Co-receptors Reporter genes    

We are well-versed in working with different cellular backgrounds such as established cancer cell lines and primary cells. Hard-to-transfect cell lines required for binding studies, functional assays, immunization in vitro or in vivo screening and efficacy evaluation applications are successfully engineered to achieve the desired attributes.    

For biologics programs, we have experience of product development for mAbs, immune-fusion molecules and cytokines. The typical activities includes: transfection, selection, Limiting dilution, media/feed screening, cell line characterization, research cell banking and protein production

Speak to our experts

Cell Lines

  • Membrane-bound receptor cell lines with expression calibrated clones (low, medium and high)
  • Receptor density measured and clones selected as per end-use requirement
  • Knock-In (KI), Knock-Down (KD) and Knock-Out (KO) cell lines using siRNA / shRNA and CRISPR/Cas9 or TALEN technologies
  • Single, double or multiple gene KO / KD in primary and established cell lines
  • Inducible cell line using Tet / Cumate inducible system
  • Reporter cell lines (Fluorescent protein / Luciferase)
  • Orthotopic cell line for in vivo xenograft and imaging applications
  • Production cell lines using commercially available systems such as Freedom CHO-S/CHO DG44/CHOZN and in-house developed lentiviral system
  • SARS-CoV-2 pseudo virus generation and neutralization assay

Vector Systems

pcDNA series, pLVX and pGIPZ / pTRIPZ lentiviral vector series, pCHO1.0, pOptivec, pGL and in-house modified vector systems

Transfection Methods

  • Standard (lipid/nanoparticle)
  • Electroporation
  • Transposon
  • Lentiviral transduction

Screening

  • Flow cytometry
  • Western blot
  • PA-HPLC
  • ELISA
  • Octet
  • ClonePix

Quality Control

  • Thaw test
  • Mycoplasma
  • Clonal stability
  • Receptor density estimation
  • SE-HPLC
  • LC-MS analysis

Why Aurigene Pharmaceutical Services?

Strong framework data recording and traceability

Recombinant cell lines for wide protein classes

In-house lentiviral platform for difficult to express reagent proteins

Extensive experience working with various vector or cell line systems

Virtual Tour

 
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