We provide a wide range of antibiotic susceptibility testing services such as Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests in accordance with the guidelines issued by the Clinical and Laboratory Standards Institute using broth microdilution, broth macro-dilution, disk diffusion, and agar dilution methods.
Our Minimum Inhibitory Concentration (MIC) test service for New Chemical Entity (NCE) helps determine the lowest concentration of an antimicrobial agent that prevents visible growth of a microorganism under defined test conditions.
We use the MIC values to evaluate the activity of new antimicrobial agents and to determine the NCE’s susceptibilities of microorganisms. MIC is considered as the “gold standard” for determining the susceptibility of organisms to antimicrobials and is therefore used to judge the performance of all other methods of susceptibility testing.
Cultured microbes are incubated with the NCE at various concentrations and measured for the results using agar dilution or broth dilution methodology to determine at what level the MIC endpoint is established. Agar dilution involves incorporating different concentrations of the antimicrobial substance into a nutrient agar medium followed by the application of a standardized number of cells to the surface of the agar plate. For broth dilution, we often determine it in 96-well microtiter plate format, where microorganisms are inoculated into a liquid growth medium in the presence of different concentrations of an antimicrobial agent. Growth is assessed after incubation for a defined period of time (16–20 hours), and until the MIC value is read.
Susceptibility testing is typically conducted using organisms that contribute to an infectious process warranting antimicrobial chemotherapy. Several microorganisms such as aerobic or anaerobic bacteria, yeasts can be tested based on the requirement.
Our Minimum Bactericidal Concentration (MBC) test services help determine the lowest concentration of an antimicrobial agent required to achieve bactericidal killing, our standards are defined at 99.9% reduction in the initial inoculum. We chose it by sub-culturing broth dilutions that inhibit the growth of a bacterial organism i.e. concentrations at and above the MIC. The broth dilutions are streaked onto agar and incubated for 24 to 48 hours. The MBC is the lowest broth dilution of antimicrobial that prevents the growth of the organism on the agar plate. Failure of the organism to grow on the plate implies that only nonviable microorganisms are present.
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