Developing a methodology for In situ perfusion based combined tissue distribution and toxicity evaluation study

The perfusate liquid was filled in calibrated batch of disposal syringes and delivered into right ventricle of the heart by means of a disposal needle fitted onto the disposable syringe. After adequately anesthetizing the animal, the perfusion process was performed using Harvard infusion pump. The vital organs (liver, lungs, spleen, brain, heart) were examined and graded for the perfusion efficiency Aurigene solution: An efficient method with optimal perfusion volume and rate was established. This accommodated estimation of analyte concentration and evaluation of microscopic changes using special staining techniques. Outcome: Assay was successfully established and launched for client offering under GLP compliance; we also received global regulatory acceptance for this assay. This assay replaces standalone assays which are time and resource consuming. Highlights: Combining two study objectives in one experimental design is challenging, but we achieved it. On-site validation was accomplished successfully, as was GLP compliance. We received recurring business as a result of this study because trust was developed with consistent efforts.