Molecular Biology Services

Recombinant DNA Technology:

• Cloning and vector generation:
  Gene cloning service at APSL supports customized generation of recombinant constructs by various methods for generation of antibodies, enzymes and reagent proteins;
  • Ligation dependent and independent cloning
  • Recombination based high throughput cloning
  • De novo cloning (Synthetic gene fragments, PCR and cDNA based)
  • Customized vector construction based on client requirement
• Plasmid DNA preparation:
  Plasmid DNA purification service caters to both small research facilities as well as large-scale manufacturing for biotech and pharmaceutical companies;
  • Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production.
  • High quality industrial grade plasmid suitable for protein, antibody production, stable cell line generation in mammalian cells, bacmid generation for protein expression in insect cells, viral packaging, in vitro mRNA synthesis, vaccine, gene therapy studies and animal immunization.
  • Process development for customized plasmid propagation and purification.
• Mutagenesis:
  Site-directed mutagenesis service enables life science researcher's by creating specific DNA mutations for studying gene regulation, DNA-protein interactions, protein structure/function relationship, enzyme function and novel recombinant proteins;
  • Point mutation, insertions, deletion or gene fragment replacements.
  • Customized mutagenesis requirements such as random and site-directed mutagenesis and mutant library generation.
  • Gene shuffling, combinatorial mutagenesis, alanine scanning and site saturation mutagenesis.

Antibody gene sequencing and reformatting:

Antibody sequence information from hybridoma is essential for protection, preservation and optimization of monoclonal antibodies (mAbs). Antibody gene sequencing and reformatting at Aurigene is a one-stop solution for recombinant expression and production of antibodies;

• RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation.
• Degenerate oligos.
• De novo antibody sequencing and analysis using LC-MS/MS.
• Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, VHH/Nanobody.
• Antibody gene sequencing for Mouse, Rat, Rabbit and other species.

Strain engineering:

Experienced in genome scale modification of bacterial and yeast strains for production of recombinant proteins, platform chemicals and metabolic engineering.

• BSL-1 and BSL-2 microbes.
• Gene knock out and knock-in using homologous recombination based methods.
• Characterization of engineered strains using microbiological methods, RT-PCR and advanced analytics.
• CRISPR-Cas9 based mutagenesis.

Library generation and evaluation:

Constructing cDNA libraries, phage libraries and yeast libraries along with provisions for high throughput screening and functional characterization.

Gene expression analysis:

Regulation and expression of genes at the transcriptional and translational stages play a crucial role in determining gene function. Scientists at APSL carry out routine expression studies by several methods;

• Quantitative real time PCR.
• ELISA in various formats.
• Flow Cytometry.
• Western blotting.
• Mass Spectrometry.

Residual protein, DNA and Mycoplasma detection:

Residual host-cell proteins (HCP) and host-cell DNA (HCD) are impurities that remain in a recombinant biotherapeutics. We ensures that these impurities and contaminants are detected and reduced to levels below those guided by regulatory agencies.

• Quantitative Real time PCR
• ELISA
• SPR (Biacore)
• Mycoplasma test

Infrastructure and facilities:

The state-of-the-art laboratory equipped with high-end instrumentation supported by experienced IT and engineering teams, at Aurigene supports discovery and development services under biology capabilities.

• Real-Time PCR, Gradient PCR instruments, Gene Amp PCR system
• Gel Documentation Systems
• Nano Drop Spectrophotometer.
• Pulsed field electrophoresis
• Bench Top Fermenters
• Biosafety Cabinets- BSL I & II
• Static Incubators and Shakers
• Wave Bioreactor
• Flow cytometer
• LiCoR Imaging system
• Biorad Molecular Imager
• Nikon Eclipse E800- Inverted light microscope
• Shimadzu UV spectrophotometer
• Beckman DU 640 spectrophotometer
• Perkin Elmer Victor X5 2030 multilabel reader
• Biotek microplate reader
• Photo multiplier detection system