The in vivo Micronucleus Assay (in vivo MNT) is a reliable and commonly employed to meet the regulatory requirements while submitting IND, to screen the compounds (chemicals, impurities, pesticides, insecticides, food and feed additives etc) for their clastogenic potential in bone marrow of rodents (Rats/Mice) and as a component of exposure-based risk assessment. This assay plays a vital role to add weight of evidence in the hazardous measurement of any chemical and quantitative risk assessments.
Young & healthy Rats/Mice will exposed to the compound of interest at different concentrations derived from dose range finding study. Post treatment, animals will be euthanized and femurs will collected. Bone marrow cells from femurs will be isolated and fixed on slides and then staining. 4000 polychromatic erythrocytes per each animal will be screened to measure the frequency of micronulated - polychromatic erythrocytes.
Positive results demonstrated as An increase in the frequency of micronucleated polychromatic erythrocytes in treated animals is an indication of induced chromosome damage. Increase can be statistically significant and/or dose-dependent.
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