We provide customized mRNA synthesis services tailored for applications ranging from screening studies to preclinical research. Our high-quality mRNA transcripts are well-suited for functional assays, therapeutics, diagnostics, and antibody production.
When chemical synthesis is not feasible, in vitro transcription (IVT) remains the preferred method for producing long, stable RNA molecules such as mRNA, saRNA, or circular RNA. Regardless of production scale, all mRNA products undergo stringent in-process and final quality control using a combination of standard and custom analytical methods. Our workflows are designed for flexibility and can be adapted to meet specific client requirements. Our mRNA offerings are fully integrated with high-quality plasmid DNA production, essential processing enzymes, and reagent generation. We support high-throughput gene synthesis with codon optimization and cloning services. Additionally, we handle E. coli clone development, cell banking, fermentation, and plasmid purification.
mRNA Formulation:
We also offer support for small-scale mRNA formulation development and characterization, enabling a variety of downstream applications.
In vitro transcription
- Sequence design, gene synthesis, and cloning
- RNA transcripts from plasmids, PCR products, and cDNA
- Conventional and Self-amplifying RNA (SaRNA), circular RNA
- Flexibility to choose transcriptional promoter (T7 or SP6)
- Normal unmodified mRNA synthesis
- Use of modified bases in mRNA transcripts (Pseudo UTP etc.)
- Several strategies for 5’ capping such as co-translational capping with Anti Reverse Cap Analog (ARCA)
- Co-translational capping by ARCA, CleanCap, and Post-translational vaccinia capping system
- Options to choose from Cap0, Cap1, or Cap2
- Template-derived poly A tail or enzymatic incorporation in the IVT mRNA template
- Optimization of mRNA translation and stability
mRNA Purification
- Well-characterized in RNAse free environment
- mRNA purification using Reverse phase, affinity, and IEX methods.
- Method optimization for gram quantity purifications
Plasmid and mRNA analytics
- Nanodrop (A260/A280)
- Purity, integrity & length confirmation (TapeStation)
- Sequence confirmation by Sanger sequencing
- Sequence confirmation by cDNA synthesis
- Residual Genomic DNA (Quantitative PCR)
- Residual host RNA (TapeStation and AGE - SYBR Gold)
- Capping efficiency and polyA tail analysis by LC-MS
- Endotoxin measurement
- SE-HPLC, RP-HPLC analysis
- Residual protein content
- Bioburden test as per USP 23
- Residual plasmid analysis (qRT-PCR)
- dsRNA analysis (ELISA)
- Immunogenicity testing for vaccine candidates
mRNA-LNP Formulation analytics
- Purity & Length confirmation (TapeStation)
- Encapsulation efficiency
- Sie and PDI (DLS)
- Zeta potential (DLS)
- Lipid content (HPLC)
- Endotoxin measurement
- Potency and expression analysis in different cell lines
- Stability analysis
Why Aurigene Pharmaceutical Services?
Customized screening and scaling up strategies
In vitro transcription, purification and quality control
Customized formulation
Modernized state-of-the-art facility
Adaptable, scalable and high-quality
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Connect with our scientific experts for your drug discovery, development, and manufacturing needs
We understand that clear communication is essential to successful collaborations, and that's why we have a dedicated team that is always ready to help you. Whether you have questions about our services, want to discuss a potential partnership, or simply want to learn more about our company, we're here to help.
Our team of experts is dedicated to providing personalised solutions tailored to your unique needs. So, please don't hesitate to reach out to us. We look forward to hearing from you and helping you achieve your business goals.
Learning Resources
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A convenient and one-pot synthesis of tetracyclic isoindolo [1,2-a]quinazoline derivatives via Lewis acid mediated sequential C–N bond formation reactions is reported. This protocol provides a simple and rapid strategy for the synthesis of 12-benzylidene-10,12-dihydroisoindolo[1,2-b]quinazoline derivatives. However, a variety of tetracyclo indole fused quinazol...
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