Empowering Peptide Innovation

Aurigene’s peptide development infrastructure is globally distributed and purpose-built for end-to-end services:

• Bangalore (Discovery Centre): Focuses on early-stage peptide discovery, equipped with solid solution or hybrid phased synthesis, HPLC systems with UV, RI, CAD, ELSD detector, Supercritical Fluid Chromatography (SFC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), reverse phase columns and lyophilizers.
• Cambridge (R&D Centre): Houses CEM Liberty Blue and Liberty Pro microwave synthesizers, high and low pressure chromatography systems with columns ranging from 10mm-150mm, isolation and lyophilization units for advanced development.
• Hyderabad (Process Development): Specializes in scaling complex peptides (up to 40 amino acids), including multiple disulfide bridges, head to tail, and side to side cyclizations, with lab-scale operations up to 1 kg. The facility is equipped with state of the art analytical instrumentation, automated SPPS synthesizers and downstream purification and isolation systems.
• Vizag (Manufacturing Site): A large-scale cGMP manufacturing site designed to support late-phase clinical and commercial peptide production. It has a peptide production capacity of 350 kg per annum, with further expansion underway. The facility is equipped with 36 reactors (15L each) of microwave assisted CEM Liberty Pro synthesizers, 80L automated and 200L manual SPPS peptide synthesizers along with 1KL automated synthesizer under installation for future scale-up. The facility also houses Reverse Phase HPLC columns ranging from 150 mm to 1200 mm, Ion exchange chromatography systems, Multi-column Counter-current Solvent Gradient Purification/MCSGP (Contichrom TWIN) for continuous purification. This site is inspected by major regulatory bodies such as USFDA, MHRA and PMDA.

We can manufacture various types of peptide classes with linear peptides up to 40 Amino Acid and peptide conjugates with small molecules, lipids, carbohydrates & PEG. Each site is supported by state-of-the-art analytical instrumentation and scalable infrastructure.

Aurigene employs a comprehensive suite of synthesis technologies to meet diverse project needs:

• Microwave-Assisted SPPS (CEM): Offers rapid synthesis, >99% coupling efficiency, and high crude purity. Ideal for complex peptides and scalable without process changes.
• Solid-Phase Peptide Synthesis (SPPS): Manual and automated platforms (e.g., CS Bio 936S) for custom synthesis with various modifications.
• Liquid-Phase Peptide Synthesis (LPPS): Suitable for small peptides (<8 amino acids) and amino acid building blocks, supporting multi-kilo scale production. It’s a cost effective approach for small peptides or linkers.
• Recombinant Peptide Production: Under development, with capabilities in microbial expression, fermentation, and downstream purification.

Our Purification capabilities include:

• Multi-column Counter-current Solvent Gradient Purification/MCSGP (Contichrom TWIN): Enables continuous purification for high-throughput and consistent quality.
• Reverse Phase HPLC: Columns ranging from 150 mm to 1200 mm diameter for both development and large-scale purification.
• Ion Exchange Chromatography: Integrated into both lab and manufacturing workflows.
• Mass-Based and Detector-Driven Systems: Including UV, RI, CAD, ELSD, and LC-MS/MS for precise monitoring and control.

Our Lyophilization Infrastructure equips freeze dryers at following scale :

• Development scale: Buchi (36-port), Martin Christ (24-port), Labconco units.
• Manufacturing scale: Multiple 200L lyophilizers at Vizag for large batch processing.
• Spray Drying and Isolation: Available for specific peptide formats and recombinant products.
• Tray and Tabletop Lyophilizers: For flexible batch sizes and custom requirements.

These capabilities ensure that peptides are purified to the highest standards and stabilized effectively for downstream applications, including clinical and commercial use.