Molecular Biology Services

Recombinant DNA Technology

• Cloning and vector generation
  
  We support you with our gene cloning service to customize the generation of recombinant constructs such as antibodies, enzymes, and reagent proteins. Our methods include:
  • Ligation dependent and independent cloning
  • Recombination based high throughput cloning
  • De novo cloning (Synthetic gene fragments, PCR and cDNA based)
  • Customized vector construction based on client requirement


• Plasmid DNA preparation
  
  Our plasmid DNA purification service caters to both small research facilities, biotechs and larger pharmaceutical companies.
  • Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production
  • High quality industrial grade plasmid suitable for protein, antibody production, stable cell line generation in mammalian cells, bacmid generation for protein expression in insect cells, viral packaging, in vitro mRNA synthesis, vaccine, gene therapy studies and animal immunization
  • Process development for customized plasmid propagation and purification
  • Large scale manufacturing


• Mutagenesis
  
  We offer site-directed mutagenesis services and support our clients by creating specific DNA mutations to study gene regulation, DNA-protein interactions, protein structure/function relationship, enzyme function, and novel recombinant proteins.
  • Point mutation, insertions, deletion, or gene fragment replacements
  • Customized mutagenesis requirements such as random and site-directed mutagenesis and mutant library generation
  • Gene shuffling, combinatorial mutagenesis, alanine scanning, and site saturation mutagenesis.

Antibody Gene Sequencing and Reformatting

Antibody sequence information from hybridoma is essential for protecting, preserving, and optimizing monoclonal antibodies (mAbs). Antibody gene sequencing and reformatting at Aurigene is a one-stop solution for recombinant expression and production of antibodies. Our offering includes:

• RACE (Rapid amplification of cDNA ends) using template switching adapter ligation
• Degenerated oligos
• De novo antibody sequencing and analysis using LC-MS/MS
• Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, VHH/nanobody
• Antibody gene sequencing for mouse, rat, rabbit and other species

Strain engineering

Our experienced team can support you wtih genome-scale modification of bacterial and yeast strains for the production of recombinant proteins, platform chemicals, and metabolic engineering.

• BSL-1 and BSL-2 microbes
• Gene knock-out and knock-in using homologous recombination-based methods
• Characterization of engineered strains using microbiological methods, RT-PCR, and advanced analytics
• CRISPR-Cas9 based mutagenesis

Library generation and evaluation

Our clients have access to our cDNA, phage, and yeast libraries which we apply for high throughput screening and functional characterizations.

Gene expression analysis

Regulation and expression of genes at transcriptional and translational stages play a crucial role in determining gene functions. We can carry out routine expression studies by several methods including:

• Quantitative real-time PCR
• ELISA in various formats
• Flow cytometry
• Western blotting
• Mass spectrometry

Residual protein, DNA and mycoplasma detection

Residual host-cell proteins (HCP) and host-cell DNA (HCD) are impurities in recombinant biotherapeutics, which need to be detected and quantified to prove that they meet the limits set by global regulatory agencies. To do that, we apply the following methods:

• Quantitative real-time PCR
• ELISA
• SPR (Biacore)
• Mycoplasma test