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In-Vitro Assay Development

Aurigene Pharma Services has vast experience and varied expertise in the in-vitro assay development and hence would offer efficient, accurate and reliable services, tailored to specific client or regulatory requirements.

The in-vitro assay development team is committed to serve our clients utilizing their experience in assay development, optimization and validation of biochemical and cellular assays to profile and rank order the potency of test compounds. We have established assays to validate drug targets, and screen small molecules for multiple therapeutic areas namely cancer, inflammatory diseases and metabolic disorders. Our experts can customise and provide specifically tailored services to clients utilizing a wide range of technologies namely absorbance, luminescence, fluorescence, fluorescence polarization and Fluorescence Resonance Energy Transfer (FRET), TR-FRET and HTR Fusing 96 and 384 well plates in a medium-throughput fashion.

 

Biochemical assays for various targets:

  • Kinase profiling using a Large panel of kinases
  • Proteases
  • Phosphatases
  • Nuclear hormone receptors
  • GPCRs
  • Dehydrogenases
  • Deacetylases and demethylases

Our array of Cell based assays:

  • Cell Migration and Invasion (Trans-well)
  • Tube Formation (In-vitro  Angiogenesis)
  • Differentiation assay
  • MTT (Cytotoxicity/Proliferation)
  • ATP synthesis assay
  • LDH
  • Cell apoptosis (Early Detection) – Annexin V
  • Cell apoptosis (Early detection) – Caspase Assay
  • PARP cleavage
  • DNA fragmentation
  • Cell cycle analysis (PI Staining)
  • Gene and protein expression study with small molecules (qRT-PCR & Western Blot)
  • Cell based reporter assays to track the effect of small molecules.